Weisser H, Witzel J G
Institute of Clinical Chemistry, University Clinic Bergmannsheil, Bochum, Germany.
Invasion Metastasis. 1993;13(4):195-200.
In vivo studies on osteosarcoma are limited by the relative low incidence of this tumor in man. Therefore, there is a high dependence on a reliable experimental tumor model. In previous studies, a transplantable osteosarcoma of the athymic nude rat was established. In the present study the content and pattern of distribution of immune effector cells within this osteosarcoma were investigated by immunohistochemistry, and were related to histomorphologic findings. The absence of T-like cells within the tumor of 1- to 2-month-old athymic nude rats was shown using the T-lymphocyte-specific monoclonal antibody OX-19. In contrast, OX-6-positive cells, representing B lymphocytes and macrophages, were distributed throughout the whole tumor with a discrete accumulation at the borderline between the peripheral proliferative tumor tissue and the central necrotic area. Using the macrophage-specific monoclonal antibody ED1 we found the accumulation of mononuclear cells to consist mainly of macrophages. The distribution of these macrophages was closely related to histomorphologic tumor characteristics: the major proportion was found in the proliferative peripheral part of the tumor with highest macrophage content at the border to the central necrosis. As the measurement of tumor necrosis in osteosarcoma plays an important role in the judgement of therapeutic response, this distinct delimitation of tumor necrosis by macrophages could be helpful in the evaluation of therapeutic effects.(ABSTRACT TRUNCATED AT 250 WORDS)
骨肉瘤的体内研究受到该肿瘤在人类中相对低发病率的限制。因此，高度依赖可靠的实验性肿瘤模型。在先前的研究中，建立了无胸腺裸鼠的可移植骨肉瘤模型。在本研究中，通过免疫组织化学研究了该骨肉瘤内免疫效应细胞的含量和分布模式，并将其与组织形态学发现相关联。使用T淋巴细胞特异性单克隆抗体OX-19显示，1至2月龄无胸腺裸鼠的肿瘤内不存在T样细胞。相比之下，代表B淋巴细胞和巨噬细胞的OX-6阳性细胞分布于整个肿瘤，在周边增殖性肿瘤组织与中央坏死区域之间的边界处有离散性聚集。使用巨噬细胞特异性单克隆抗体ED1，我们发现单核细胞的聚集主要由巨噬细胞组成。这些巨噬细胞的分布与肿瘤组织形态学特征密切相关：主要部分位于肿瘤的增殖性周边部分，在与中央坏死区域的边界处巨噬细胞含量最高。由于骨肉瘤中肿瘤坏死的测量在治疗反应的判断中起重要作用，巨噬细胞对肿瘤坏死的这种明显界定可能有助于评估治疗效果。（摘要截短于250字）
