Birdsell D C, Doyle R J, Morgenstern M
J Bacteriol. 1975 Feb;121(2):726-34. doi: 10.1128/jb.121.2.726-734.1975.
The phytohemagglutinin, concanavalin A (Con A), interacts specifically and reversibly with the polyglucosyl glycerol phosphate teichoic acid of Bacillus subtilis 168 cell walls. Advantage has been taken of this interaction to examine the organization of the surface teichoic acid at the ultrastructural level. Con A-treated whole cells and cell walls contain an irregular, fluffy layer 25 to 60 nm thick which is absent in untreated or alpha-methyl glucoside-treated preparations. This discontinuous layer is present only on the outer profile of Con-A-treated cell walls. The surface teichoic acid is proposed to be oriented perpendicular to the long axis of the cell. Fixation and embedment for electron microscopy result in condensation of this layer which then contributes to the stainable portion of the wall. Con A treatment binds adjacent teichoic acid molecules in their native configuration producing the irregular, fluffy layer visualized.
植物血凝素、伴刀豆球蛋白A（Con A）与枯草芽孢杆菌168细胞壁的聚葡糖基甘油磷酸壁酸特异性且可逆地相互作用。利用这种相互作用在超微结构水平上研究表面壁酸的组织。经Con A处理的完整细胞和细胞壁含有一层25至60纳米厚的不规则蓬松层，未处理或经α-甲基葡糖苷处理的制剂中不存在该层。这种不连续层仅存在于经Con A处理的细胞壁的外表面。表面壁酸被认为垂直于细胞的长轴定向。电子显微镜的固定和包埋导致该层浓缩，然后这部分成为细胞壁可染色的部分。Con A处理以其天然构型结合相邻的壁酸分子，产生可见的不规则蓬松层。
