农民肺疾病患者对普通嗜热放线菌抗原的抗体反应。
Antibody responses in patients with farmer's lung disease to antigens from Thermoactinomyces vulgaris.
作者信息
Hollingdale M R
出版信息
J Hyg (Lond). 1975 Feb;74(1):35-41. doi: 10.1017/s0022172400046696.
A serological analysis of mycelial antigens of Thermoactinomyces vulgaris in immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid. Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies to T. vulgaris; the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.
对寻常嗜热放线菌的菌丝体抗原与人血清进行免疫扩散血清学分析，发现了五种独立的抗原。三种抗原是蛋白质，对链霉蛋白酶敏感且可溶于苯酚。两种是阳离子多糖，对高碘酸钠敏感，含有葡糖胺和胞壁酸。将包被有菌丝体抗原的乳胶与沉淀素试验在检测针对寻常嗜热放线菌的抗体方面进行比较；每种试验检测到的阳性结果数量略有不同，两种试验结合检测到的数量最多。对流免疫电泳（CIE）被证明是检测沉淀素的非常灵敏的方法，但不适用于其定量测定。建议对免疫扩散、乳胶凝集和CIE作为农民肺疾病潜在血清诊断技术进行前瞻性评估。
Zotero 插件