Aguilar-Santelises M, Mellstedt H, Jondal M
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Leukemia. 1994 Jul;8(7):1146-52.
Isolated leukemic B cells from patients with B-chronic lymphocytic leukemia (B-CLL) were tested for proliferative response in vitro to Staphylococcus Aureus strain Cowan 1 (SAC), IL-2, and low molecular weight (MW) BCGF. Patients were classified according to clinical stage and progressiveness. Ten of eighteen cell populations from patients with progressive B-CLL responded in vitro with a stimulation index (SI) > 20. Only 1/16 non-progressive patients had a proliferative but low response. Normal unfractionated tonsillar B cells responded to SAC and BCGF, whereas normal high buoyant density B cells were unresponsive. After 3 days of stimulation, responding B-CLL cells had multiplied and the B cells expressed CD5, CD19, and weakly CD21. No cells in the responding cultures exhibited CD3 or the EBV nuclear antigen EBNA-1. Cell maturation, measured as IgM secretion, was found in some, but not in all responding B-CLL cultures. Thus, B-CLL cells from patients with progressive disease have the capacity to respond to signaling through surface Ig receptors and to certain T-cell factors which was not the case for B-CLL cells from non-progressive patients. The pattern of in vitro response may be related to disease progression, reflecting a dependency of normal immunoregulatory mechanisms and/or a dysregulation of the growth control in the leukemic cells.
对来自B - 慢性淋巴细胞白血病（B - CLL）患者的分离白血病B细胞进行体外试验，检测其对金黄色葡萄球菌考恩1株（SAC）、白细胞介素 - 2（IL - 2）和低分子量（MW）B细胞生长因子（BCGF）的增殖反应。根据临床分期和病情进展对患者进行分类。来自进展性B - CLL患者的18个细胞群体中有10个在体外有反应，刺激指数（SI）> 20。只有1/16的非进展性患者有增殖但反应较弱。正常未分离的扁桃体B细胞对SAC和BCGF有反应，而正常高浮力密度B细胞无反应。刺激3天后，有反应的B - CLL细胞增殖，这些B细胞表达CD5、CD19，且弱表达CD21。反应培养物中的细胞均未表现出CD3或EB病毒核抗原EBNA - 1。在一些但并非所有有反应的B - CLL培养物中发现了以IgM分泌衡量的细胞成熟情况。因此，进展性疾病患者的B - CLL细胞有能力对通过表面Ig受体的信号传导以及某些T细胞因子产生反应，而非进展性患者的B - CLL细胞则不然。体外反应模式可能与疾病进展有关，反映了正常免疫调节机制的依赖性和/或白血病细胞生长控制的失调。
