Perez J E, Ogusuku E, Inga R, Lopez M, Monje J, Paz L, Nieto E, Arevalo J, Guerra H
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima.
Trans R Soc Trop Med Hyg. 1994 Mar-Apr;88(2):161-4. doi: 10.1016/0035-9203(94)90276-3.
Natural infection of Lutzomyia spp. with Leishmania was studied with the aid of the polymerase chain reaction (PCR) in Chaute, Lima, Perú, a locality endemic for Andean cutaneous leishmaniasis (uta). The PCR, with primers specific for the L. braziliensis complex, was applied to sandfly pools. Sandflies were sampled from April 1990 to May 1991 with CDC light traps in homes, and from near homes with a Shannon trap using protected human bait. Lu. verrucarum (4 pools) and Lu. peruenis (2 pools) from the anthropophilic collections, and Lu. verrucarum (2 pools) from indoors were found to be infected with Leishmania. The majority of infected sandflies were recorded mainly in April 1991 (4 pools), coinciding with the highest sandfly densities and the maximum number of new cases of uta (7). Non-infected sandflies were found from May to October 1990 and January to March 1991. Thus, these 2 sandfly species play a role in the spread of leishmaniasis among humans and other animals in Chaute.
在秘鲁利马的绍特，借助聚合酶链反应（PCR）对罗蛉属（Lutzomyia spp.）自然感染利什曼原虫的情况进行了研究，该地是安第斯皮肤利什曼病（uta）的地方性流行区。使用针对巴西利什曼原虫复合体的特异性引物，对沙蝇样本池进行PCR检测。1990年4月至1991年5月期间，在家中使用疾控中心（CDC）诱虫灯诱捕沙蝇，并在住家附近使用装有防护装置的人体诱饵，通过香农诱捕器采集沙蝇。在嗜人习性采集的样本中，疣罗蛉（Lu. verrucarum，4个样本池）和秘鲁罗蛉（Lu. peruenis，2个样本池）以及室内采集的疣罗蛉（2个样本池）被发现感染了利什曼原虫。大多数受感染的沙蝇主要记录于1991年4月（4个样本池），这与沙蝇密度最高以及uta新发病例数最多（7例）的时间相吻合。在1990年5月至10月以及1991年1月至3月期间发现了未感染的沙蝇。因此，这两种沙蝇在绍特地区利什曼病在人类和其他动物之间的传播中发挥了作用。
