Kato Hirotomo, Cáceres Abraham G, Hashiguchi Yoshihisa
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Sección de Entomología, Instituto de Medicina Tropical "Daniel A. Carrión" y Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Peru.
PLoS Negl Trop Dis. 2016 Jan 6;10(1):e0004336. doi: 10.1371/journal.pntd.0004336. eCollection 2016 Jan.
The natural infection of sand flies by Leishmania was examined in the Department of Huanuco of Peru, where cutaneous leishmaniasis caused by a hybrid of Leishmania (Viannia) braziliensis/L. (V.) peruviana is endemic. A total of 2,997 female sand flies were captured by CDC light traps and Shannon traps, of which 2,931 and 66 flies were identified as Lutzomyia tejadai and Lu fischeri, respectively. Using crude DNA extracted from individual sand flies as a template, Leishmania DNA was detected from one Lu. tejadai. The parasite species was identified as a hybrid of L. (V.) braziliensis/L. (V.) peruviana on the basis of cytochrome b and mannose phosphate isomerase gene analyses. The result suggested that Lu. tejadai is responsible for the transmission of the hybrid Leishmania circulating in this area.
在秘鲁瓦努科省对沙蝇自然感染利什曼原虫的情况进行了检查，该地区由巴西利什曼原虫（维氏亚属）/秘鲁利什曼原虫（维氏亚属）杂交种引起的皮肤利什曼病呈地方性流行。通过疾控中心诱虫灯和香农诱捕器共捕获2997只雌性沙蝇，其中2931只和66只沙蝇分别被鉴定为特氏罗蛉和费氏罗蛉。以从单个沙蝇中提取的粗DNA为模板，在1只特氏罗蛉中检测到利什曼原虫DNA。基于细胞色素b和甘露糖磷酸异构酶基因分析，该寄生虫物种被鉴定为巴西利什曼原虫（维氏亚属）/秘鲁利什曼原虫（维氏亚属）的杂交种。结果表明，特氏罗蛉是该地区传播的杂交利什曼原虫的传播媒介。
