Trees A J, Guy F, Low J C, Roberts L, Buxton D, Dubey J P
Liverpool School of Tropical Medicine, University of Liverpool.
Vet Rec. 1994 Apr 16;134(16):405-7. doi: 10.1136/vr.134.16.405.
By means of an immunofluorescence antibody test (IFAT), using in vitro cultured parasites as antigen, antibodies to Neospora species at titres > or = 1/1280 were found in 11 of 120 Scottish cattle that had recently aborted but in only one of 97 cattle from herds in which there had been no recent abortions (P < 0.01). The specificity of the antibodies was confirmed by the lack of cross reactivity between samples with high titres to Neospora and toxoplasma antigen in a direct agglutination test, and by the absence of reactivity at > or = 1/640 in the IFAT of convalescent sera from cattle infected experimentally with Toxoplasma gondii, Sarcocystis cruzi, Eimeria bovis, E alabamensis, Cryptosporidium parvum and Babesia divergens. These results demonstrate that Neospora species infection occurs commonly in aborting cattle in Britain, and that the IFAT may be a useful tool for investigating the infection.
通过免疫荧光抗体试验（IFAT），以体外培养的寄生虫作为抗原，在120头近期流产的苏格兰牛中，有11头检测到抗新孢子虫属的抗体效价≥1/1280，而在97头来自近期无流产情况牛群的牛中，只有1头检测到该抗体（P<0.01）。通过在直接凝集试验中高滴度抗新孢子虫样本与弓形虫抗原之间缺乏交叉反应，以及在实验感染刚地弓形虫、克氏肉孢子虫、牛艾美耳球虫、阿拉巴马艾美耳球虫、微小隐孢子虫和分歧巴贝斯虫的牛恢复期血清的IFAT中，效价≥1/640时无反应，证实了抗体的特异性。这些结果表明，新孢子虫属感染在英国流产牛中普遍存在，并且IFAT可能是调查该感染的有用工具。
