Green C, Daniels G, Skov F, Tippett P
MRC Blood Group Unit, London, UK.
Vox Sang. 1994;66(3):237-41.
Red cells carrying the low-frequency MNS antigen Mg reacted with the only example of anti-DANE, an antibody which had previously defined the GP. Dane (Mi.IX) phenotype. Furthermore, Mg+ cells reacted with the original anti-Mur (serum of Mrs. Murrell), but with none of 14 other anti-Mur. Therefore, Mg+ cells carry both DANE antigen and an atypical Mur antigen. Immunoblotting of membranes from Mg+ cells with anti-M, and with eluates prepared from anti-Mg and Mrs. Murrell's serum demonstrated a glycophorin A (GPA) molecule whose mobility was increased by an apparent M(r) of about 3,000 presumably due to the loss of the three O-glycans known to be absent from Mg-active GPA.
携带低频MNS抗原Mg的红细胞与抗DANE（一种先前确定了GP.Dane（Mi.IX）表型的抗体）的唯一实例发生反应。此外，Mg+细胞与原始抗Mur（默雷尔夫人的血清）发生反应，但与其他14种抗Mur均无反应。因此，Mg+细胞同时携带DANE抗原和一种非典型的Mur抗原。用抗-M、从抗-Mg和默雷尔夫人血清制备的洗脱液对Mg+细胞膜进行免疫印迹分析，结果显示一种血型糖蛋白A（GPA）分子，其迁移率增加了约3000的表观相对分子质量，这可能是由于Mg活性GPA中已知不存在的三个O-聚糖缺失所致。
