Moore R, Mathison G E
Department of Biology, U.W.I., Cave Hill, Barbados.
West Indian Med J. 1994 Mar;43(1):7-8.
Fourteen freshwater or brackish-water samples taken from different sites were examined for the presence of Vibrio cholerae. Standard enrichment techniques, using pre-incubation in alkaline peptone water and plating on thiosulfate citrate bile sucrose agar (TCBS) followed by biochemical, physiological and morphological characterization of the isolates, revealed the presence of Vibrio cholerae at nine of the sites examined. Serotyping for type 01 only was performed. All the strains isolated were non-01 Vibrio cholerae.
对从不同地点采集的14份淡水或微咸水样本进行了霍乱弧菌检测。采用标准富集技术,先在碱性蛋白胨水中预培养,然后接种于硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂(TCBS)平板上,随后对分离株进行生化、生理和形态学特征鉴定,结果显示在所检测的9个地点存在霍乱弧菌。仅进行了01型血清分型。所有分离出的菌株均为非01群霍乱弧菌。