Marsh R F, Hanson R P
Science. 1975 Feb 21;187(4177):656. doi: 10.1126/science.803711.
Corneal epithelium from hamsters dying of transmissible mink encephalopathy contained a virus titer of 10-4.8 times the 50 percent lethal dose (10-4.8 LD50) per 0.05 milliliter when assayed as a cell suspension derived directly from the infected animal. After one passage in tissue culture, an equivalent concentration of cells contained only 10-0.8 LD50 per 0.05 milliliter.. It is concluded that corneal tissues are infectious; the infectivity may be mainly associated with free nerve endings. However, the most important immediate inference is that corneas from human beings affected with Creuzfeldt-Jakob disease are likely to be lethal if transplanted to healthy recipients.
死于传染性水貂脑病的仓鼠角膜上皮，经检测作为直接从受感染动物获得的细胞悬液时，每0.05毫升含有病毒滴度为50%致死剂量的10-4.8倍（10-4.8 LD50）。在组织培养中传代一次后，同等浓度的细胞每0.05毫升仅含有10-0.8 LD50。得出的结论是角膜组织具有传染性；这种传染性可能主要与游离神经末梢有关。然而，最重要的直接推断是，患有克雅氏病的人类角膜如果移植给健康接受者可能是致命的。
