Romp K G, Peters W P, Hoffman M
Department of Pathology, Durham Veterans Affairs Medical Center, NC 27705.
Am J Hematol. 1994 Aug;46(4):319-24. doi: 10.1002/ajh.2830460411.
Thiazole orange (TO) is a fluorescent dye that is commonly used for flow cytometric measurement of erythrocytic reticulocytes. This technique has also been validated for counting "reticulated" platelets, as a measure of bone marrow thrombopoietic activity. Patients with an established diagnosis of idiopathic thrombocytopenic purpura (ITP) have been reported to have a three- to five-fold increase in the percent of circulating reticulated platelets. The current study was conducted to determine if platelet reticulocyte counts predicted recovery of bone marrow thrombopoietic activity following autologous bone marrow transplantation. We found an increase in the percent of circulating reticulated platelets preceding recovery of the platelet count; then, as the patients' platelet counts rose, the percent reticulated platelets fell. In patients with prolonged thrombocytopenia, a persistent increase in the proportion of reticulated platelets suggested a consumptive process, as opposed to failure of engraftment. Moderate transfusion of platelet concentrates did not interfere with the ability of the platelet reticulocyte measurements to detect increased thrombopoietic activity. Platelet transfusions did obscure any decrease in the proportion of reticulated platelets. Our results show that platelet reticulocyte counts could be useful in monitoring the recovery of marrow function following autologous bone marrow transplantation.
噻唑橙（TO）是一种荧光染料，常用于流式细胞术检测红细胞网织红细胞。该技术也已被验证可用于计数“网状”血小板，作为骨髓血小板生成活性的一种测量方法。据报道，确诊为特发性血小板减少性紫癜（ITP）的患者循环中网织血小板的百分比增加了三到五倍。本研究旨在确定血小板网织红细胞计数是否可预测自体骨髓移植后骨髓血小板生成活性的恢复。我们发现，在血小板计数恢复之前，循环中网织血小板的百分比增加；然后，随着患者血小板计数上升，网织血小板的百分比下降。在血小板减少持续时间较长的患者中，网织血小板比例持续增加提示存在消耗性过程，而非植入失败。适度输注血小板浓缩液并不影响血小板网织红细胞测量检测血小板生成活性增加的能力。血小板输注确实掩盖了网织血小板比例的任何下降。我们的结果表明，血小板网织红细胞计数可用于监测自体骨髓移植后骨髓功能的恢复。
