Soyombo O, Spur B W, Lee T H
Department of Allergy and Allied Respiratory Disorders, UMDS, Guy's Hospital, London, UK.
Allergy. 1994 Apr;49(4):230-4. doi: 10.1111/j.1398-9995.1994.tb02654.x.
Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10(-6) M, it achieved about 20% of the response of 10(-6) M platelet-activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10(-10)-10(-5) M) resulted in a concentration-dependent inhibition of cell migration induced by 10(-6) M formyl-methionyl-leucyl-phenylalanine (FMLP) and 10(-6) M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10(-6) M. LXA4 (10(-10)-10(-6) M) did not elicit ECP release or modulate ECP release induced by 10(-6) M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.
脂氧素是通过花生四烯酸的双加氧作用衍生而来的三羟基四烯代谢产物。脂氧素A4（LXA4）通过全化学合成制备，并对其调节嗜酸性粒细胞迁移的能力进行了评估。LXA4是一种弱的部分趋化剂；在10^(-6) M时，它达到了10^(-6) M血小板活化因子（PAF）反应的约20%。用递增剂量的LXA4（10^(-10)-10^(-5) M）对嗜酸性粒细胞进行预孵育，导致10^(-6) M甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和10^(-6) M PAF诱导的细胞迁移受到浓度依赖性抑制。产生50%嗜酸性粒细胞迁移抑制（IC50）的LXA4浓度约为10^(-6) M。LXA4（10^(-10)-10^(-6) M）不会引发嗜酸性粒细胞阳离子蛋白（ECP）释放，也不会调节10^(-6) M FMLP诱导的ECP释放。LXA4在预防嗜酸性粒细胞迁移方面可能具有抗过敏特性。
