Koike K, Sakamoto Y, Sawada T, Ohmichi M, Kanda Y, Nohara A, Hirota K, Kiyama H, Miyake A
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):161-7. doi: 10.1006/bbrc.1994.1907.
We investigated the possibility of detection of CINC/gro, which is a IL-8-like neutrophil chemoattractant, immunoreactivity in rat normal anterior pituitary gland by immunohistochemistry, western blot analysis and an ELISA. We first ascertained the possibility of detection of CINC/gro immunoreactivity in the anterior pituitary gland by immunocytochemistry. In the anterior lobe of the pituitary gland, a few CINC/gro-like immunoreactive cells were observed (1-3% of all cells in the anterior pituitary). The positive cells were middle or large in size and looked angular in shape. Intense immunoreactivity was observed in the cytoplasm but not in the nucleus. Analysis by immunoblotting with anti-CINC/gro antiserum gave a characteristic single CINC/gro band with a molecular weight of 6.3 kDa. CINC/gro immunoreactivity was also detected in 3-h conditioned medium of normal anterior pituitary cells by an ELISA, and that immunoreactivity increased significantly in a time-dependent manner during 24-h incubation. This immunoreactivity could be induced by TNF-alpha in a dose-dependent manner. These findings indicate that CINC/gro is produced in pituitary gland and also suggests the possibility that CINC/gro may play some role asa modulator of anterior pituitary function, especially in the cross-talk mechanism between the immune and neuroendocrine systems.
我们通过免疫组织化学、蛋白质印迹分析和酶联免疫吸附测定（ELISA），研究了在大鼠正常垂体前叶中检测CINC/gro（一种白细胞介素-8样中性粒细胞趋化因子）免疫反应性的可能性。我们首先通过免疫细胞化学确定了在垂体前叶检测CINC/gro免疫反应性的可能性。在垂体前叶中，观察到一些CINC/gro样免疫反应性细胞（占垂体前叶所有细胞的1%-3%）。阳性细胞中等大小或较大，形状呈角形。在细胞质中观察到强烈的免疫反应性，但在细胞核中未观察到。用抗CINC/gro抗血清进行免疫印迹分析得到一条特征性的单一CINC/gro条带，分子量为6.3 kDa。通过ELISA在正常垂体前叶细胞3小时的条件培养基中也检测到了CINC/gro免疫反应性，并且在24小时孵育期间，该免疫反应性以时间依赖性方式显著增加。这种免疫反应性可被肿瘤坏死因子-α以剂量依赖性方式诱导。这些发现表明CINC/gro在垂体中产生，也提示了CINC/gro可能作为垂体前叶功能调节剂发挥某些作用的可能性，尤其是在免疫和神经内分泌系统之间的相互作用机制中。
