Asai K, Fujisaki S, Nishimura Y, Nishino T, Okada K, Nakagawa T, Kawamukai M, Matsuda H
Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):340-5. doi: 10.1006/bbrc.1994.1933.
The gene located upstream of the nlp gene at min 69 on the chromosome of Escherichia coli was cloned from the plasmid pLC7-42 constructed by Clarke and Carbon. The extract of the strain harboring the plasmid containing this gene showed increased activity of the prenyltransferase. The product of the enzyme reaction was analyzed by two systems of the thin-layer chromatography and shown to be the phosphate ester of all-E-octaprenol. These results suggested that the gene was the structural gene for the octaprenyl diphosphate synthase which supplied the precursor of the side chain of the isoprenoid quinones.
位于大肠杆菌染色体上69分钟处nlp基因上游的基因，是从克拉克和卡尔文构建的质粒pLC7 - 42中克隆得到的。携带含有该基因质粒的菌株提取物显示，异戊烯基转移酶的活性增强。通过两种薄层色谱系统对酶反应产物进行分析，结果表明其为全-E-辛戊二烯醇的磷酸酯。这些结果表明，该基因是八异戊二烯基二磷酸合酶的结构基因，该酶为类异戊二烯醌侧链提供前体。
