Fukutomi O, Kondo N, Agata H, Shinoda S, Kuwabara N, Shinbara M, Inoue R, Orii T
Department of Pediatrics, Gifu University School of Medicine, Japan.
Clin Exp Allergy. 1994 Apr;24(4):359-66. doi: 10.1111/j.1365-2222.1994.tb00247.x.
Monocyte chemotactic activities in supernatants of ovalbumin (OA)-stimulated peripheral blood mononuclear cell (PBMC) cultures were studied in patients with atopic dermatitis (AD) who were sensitive to hen's egg. The monocyte chemotactic activities in hen's egg-sensitive AD patients were significantly higher than those of non-atopic healthy controls and patients with immediate allergic symptoms. However, the monocyte chemotactic activities were not detected in bovine serum albumin-stimulated PBMC culture supernatants in patients with AD who were sensitive to hen's egg, but not to cow's milk. Furthermore, there was significant correlation between the monocyte chemotactic activities and proliferative responses of PBMCs to OA in hen's egg-sensitive AD patients, whereas there was no significant correlation between the monocyte chemotactic activities and radioallergosorbent test values. These results suggest that PBMCs stimulated with food antigens produce monocyte chemotactic factors which relate to the pathogenesis of AD in food-sensitive AD patients and that the pathogenesis of AD may be related to cell-mediated immune responses.
在对鸡蛋敏感的特应性皮炎（AD）患者中，研究了卵清蛋白（OA）刺激的外周血单个核细胞（PBMC）培养上清液中的单核细胞趋化活性。鸡蛋敏感的AD患者的单核细胞趋化活性显著高于非特应性健康对照者和有速发型过敏症状的患者。然而，在对鸡蛋敏感但对牛奶不敏感的AD患者中，牛血清白蛋白刺激的PBMC培养上清液中未检测到单核细胞趋化活性。此外，在鸡蛋敏感的AD患者中，单核细胞趋化活性与PBMC对OA的增殖反应之间存在显著相关性，而单核细胞趋化活性与放射变应原吸附试验值之间无显著相关性。这些结果表明，食物抗原刺激的PBMC产生与食物敏感的AD患者AD发病机制相关的单核细胞趋化因子，且AD的发病机制可能与细胞介导的免疫反应有关。
