枯草芽孢杆菌多效性负向芽孢形成突变体的遗传分析。
Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis.
作者信息
Hoch J A
出版信息
J Bacteriol. 1971 Mar;105(3):896-901. doi: 10.1128/jb.105.3.896-901.1971.
Abstract
Genetic studies were undertaken on 14 pleiotropic negative sporulation mutants. These mutants (spoA) which are blocked early in the sporulation process were found to map near the terminus of the Bacillus subtilis chromosome in a region enriched in genes involved in spore formation. Two- and three-factor crosses by transduction and transformation led to the conclusion that the pleiotropic spoA mutations formed a linked cluster. The genetic distance across the cluster calculated from transformation data was compatible with the mutant sites defining a single gene. Suppressor studies revealed that either a nonsense or missense mutation in the spoA locus generated a pleiotropic negative phenotype. It was concluded that the locus codes for a protein, and the absence of this protein is responsible for the pleiotropic phenotype.
摘要
对14个多效性负孢子形成突变体进行了遗传学研究。这些在孢子形成过程早期受阻的突变体（spoA）被发现定位于枯草芽孢杆菌染色体末端附近的一个区域，该区域富含参与孢子形成的基因。通过转导和转化进行的双因子和三因子杂交得出结论，多效性spoA突变形成了一个连锁簇。根据转化数据计算出的整个簇的遗传距离与定义单个基因的突变位点相符。抑制子研究表明，spoA基因座中的无义或错义突变都会产生多效性负表型。得出的结论是，该基因座编码一种蛋白质，这种蛋白质的缺失导致了多效性表型。
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