通过Southern印迹杂交和聚合酶链反应（PCR）检测宫颈癌组织中的16型和52b型人乳头瘤病毒（HPV）

Detection of human papillomavirus (HPV) type 16 and 52b in cervical cancer tissues by Southern blot hybridization and polymerase chain reaction (PCR).

作者信息

Yaegashi N, Yajima H, Shikano K, Konno R, Sato S, Noda K, Yajima A

机构信息

Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Virus Genes. 1990 Dec;4(4):313-23. doi: 10.1007/BF00570026.

DOI:10.1007/BF00570026

PMID:1962976

Abstract

DNA samples from cancer tissues diagnosed histologically as squamous cell carcinoma of the uterine cervix were examined for the presence of human papillomavirus (HPV) genome DNA by Southern blot hybridization. Of 63 specimens, 32 were found to hybridize with HPV DNA probes; 23 specimens (35%) with HPV type 16 (HPV16), one (2%) with HPV type 18 (HPV18), four (7%) with HPV type 52b (HPV52b), and four others (7%) weakly with HPV52b. Specimens negative for HPV DNA with Southern blot hybridization were subjected to polymerase chain reaction (PCR) to determine the presence of HPV DNA under more sensitive conditions. After PCR using one set of primers specific for HPV16 and HPV52b, 7 out of 31 specimens were found to have HPV16 DNA. None was positive for HPV52b DNA. Our results indicate that HPV52b, as well as HPV16 and HPV18, is associated with squamous cell carcinoma of uterine cervix, and more sensitive determination of HPV infection can be made by amplification of the viral genome by PCR.

摘要

通过Southern印迹杂交法检测经组织学诊断为子宫颈鳞状细胞癌的癌组织DNA样本中是否存在人乳头瘤病毒（HPV）基因组DNA。在63个样本中，发现32个与HPV DNA探针杂交；23个样本（35%）与16型HPV（HPV16）杂交，1个（2%）与18型HPV（HPV18）杂交，4个（7%）与52b型HPV（HPV52b）杂交，另外4个（7%）与HPV52b弱杂交。对Southern印迹杂交HPV DNA阴性的样本进行聚合酶链反应（PCR），以在更敏感的条件下确定HPV DNA的存在。使用一组针对HPV16和HPV52b的特异性引物进行PCR后，31个样本中有7个被发现含有HPV16 DNA。没有一个样本HPV52b DNA呈阳性。我们的结果表明，HPV52b以及HPV16和HPV18与子宫颈鳞状细胞癌有关，并且通过PCR扩增病毒基因组可以更敏感地确定HPV感染情况。