Syrový I
Institute of Physiology, Czech Academy of Sciences, Prague.
J Biochem Biophys Methods. 1994 Mar;28(2):115-21. doi: 10.1016/0165-022x(94)90025-6.
Albumin was glycated (nonenzymatically glycosylated) with glucose, fructose, galactose, ribose or glyceraldehyde for 5, 9, 15 and 19 days. The extent of glycation was determined (a) by the thiobarbituric acid method, (b) by fructosamine assay, (c) by method based on the reaction with hydrazine, and (d) by measurement of fluorescence. Results show that the three colorimetric methods used differ in the sensitivity and in addition with the use of each method not the same extent of glycation with various sugars was found.
将白蛋白与葡萄糖、果糖、半乳糖、核糖或甘油醛进行糖基化（非酶促糖基化）处理5天、9天、15天和19天。通过以下方法测定糖基化程度：（a）硫代巴比妥酸法；（b）果糖胺测定法；（c）基于与肼反应的方法；（d）荧光测量法。结果表明，所使用的三种比色法在灵敏度上存在差异，此外，使用每种方法时，发现与各种糖类的糖基化程度也不尽相同。
