Mohd Dom Nur Sumirah, Yahaya Nurshieren, Adam Zainah, Nik Abd Rahman Nik Mohd Afizan, Hamid Muhajir
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Seri Kembangan, Selangor, Malaysia.
Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis, 02100 Sungai Chuchuh, Padang Besar, Perlis, Malaysia.
Evid Based Complement Alternat Med. 2020 Aug 7;2020:6374632. doi: 10.1155/2020/6374632. eCollection 2020.
The present study aimed to evaluate the potential of standardized methanolic extracts from seven varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of extracts significantly restrained the formation of fluorescence AGEs by 4.55-5.14 fold. The extracts also reduced the fructosamine levels by 47.0-86.5%, increased the thiol group levels by 64.3-83.7%, and inhibited the formation of protein carbonyl by 1.36-1.76 fold. DPPH radical scavenging activity showed an IC value of 66.81-288.04 g/ml and reducing power activity depicted at 0.02-0.24 g/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.
本研究旨在评估七个品种的标准化甲醇提取物在抑制晚期糖基化终产物（AGEs）形成、蛋白质氧化及其抗氧化作用方面的潜力。基于对AGEs、果糖胺和巯基水平的抑制，随后对蛋白质羰基形成的抑制来分析抗糖基化活性。评估了抗氧化活性（DPPH自由基清除活性和还原能力测定）和总酚含量。诱导28天后，所有品种的提取物均显著抑制荧光AGEs的形成，抑制倍数为4.55 - 5.14倍。提取物还使果糖胺水平降低了47.0 - 86.5%，使巯基水平提高了64.3 - 83.7%，并抑制蛋白质羰基的形成达1.36 - 1.76倍。DPPH自由基清除活性的IC值为66.81 - 288.04μg/ml，还原能力活性为0.02 - 0.24μg/ml。提取物中酚类化合物的含量范围为70.90至299.78mg·GAE/g。除此之外，还观察了各活性之间的相关性研究。本研究表明，七个品种具有抑制AGEs形成的潜力，并具有抗氧化活性，这可能归因于酚类化合物的存在。
