Tanaka S, Komori K, Okadome K, Sugimachi K, Mori R
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Vasc Surg. 1994 Aug;20(2):235-43. doi: 10.1016/0741-5214(94)90011-6.
We previously reported the possible role of human cytomegalovirus in the pathogenesis of inflammatory aortic diseases. To further analyze the viral cause of human aortic diseases, in this study we examined the presence and the replication of human Herpesviridae in 60 aortic tissues, including 7 inflammatory aneurysms, 37 atherosclerotic aneurysms, and 16 normal aortas.
To detect the genome of herpes simplex virus (type 1, type 2), cytomegalovirus, and Epstein-Barr virus, DNA polymerase chain reaction for each virus was performed. To analyze these herpesviral replications, the viral transcript was detected with RNA polymerase chain reaction.
The DNA polymerase chain reaction showed that either herpes simplex virus or cytomegalovirus was present more frequently in inflammatory (29% or 86%, respectively) and atherosclerotic aneurysms (27% or 65%, respectively) than in normal aortic tissues (6% or 31%, respectively), whereas the Epstein-Barr viral genome was not detected in any aortic tissue specimens. By the use of RNA polymerase chain reaction, only the cytomegaloviral transcript was recognized in 71% of the inflammatory aneurysms but was not recognized in any other tissue specimens. No other herpesviral transcripts were detected in any tissue specimens examined in this study.
Our results thus suggest that the human herpesviruses may play various roles in the pathogenicity of aortic diseases, in particular the replicating infections of the cytomegalovirus might potentially cause the formation of inflammatory aneurysms.
我们之前报道了人类巨细胞病毒在炎性主动脉疾病发病机制中的可能作用。为了进一步分析人类主动脉疾病的病毒病因，在本研究中，我们检测了60个主动脉组织中人类疱疹病毒科的存在情况及复制情况，这些组织包括7个炎性动脉瘤、37个动脉粥样硬化性动脉瘤和16个正常主动脉。
为检测单纯疱疹病毒（1型、2型）、巨细胞病毒和EB病毒的基因组，对每种病毒进行了DNA聚合酶链反应。为分析这些疱疹病毒的复制情况，用RNA聚合酶链反应检测病毒转录本。
DNA聚合酶链反应显示，单纯疱疹病毒或巨细胞病毒在炎性（分别为29%或86%）和动脉粥样硬化性动脉瘤（分别为27%或65%）中的出现频率高于正常主动脉组织（分别为6%或31%），而在任何主动脉组织标本中均未检测到EB病毒基因组。通过RNA聚合酶链反应，仅在71%的炎性动脉瘤中检测到巨细胞病毒转录本，而在其他任何组织标本中均未检测到。在本研究检测的任何组织标本中均未检测到其他疱疹病毒转录本。
因此，我们的结果表明，人类疱疹病毒可能在主动脉疾病的致病性中发挥多种作用，特别是巨细胞病毒的复制性感染可能潜在地导致炎性动脉瘤的形成。
