Jabłońska Agnieszka, Zagrapan Branislav, Paradowska Edyta, Neumayer Christoph, Eilenberg Wolf, Brostjan Christine, Klinger Markus, Nanobachvili Josif, Huk Ihor
Laboratory of Virology, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Department of Surgery, Division of Vascular Surgery, Medical University of Vienna, Vienna, Austria.
J Med Virol. 2021 Aug;93(8):5017-5024. doi: 10.1002/jmv.26901. Epub 2021 May 3.
An abdominal aortic aneurysm (AAA) is a multifactorial disease with a variety of genetic and environmental risk factors, but the exact mechanism of AAA formation and progression is still not well understood. The present study investigated the frequency of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and papillomavirus types 6 and 11 (HPV6 and HPV11), their impact on clinical manifestations of cardiovascular diseases, and their possible association with inflammation in patients with AAA and healthy volunteers. Genotyping of CMV UL75, EBV LMP-1, and HPV6, and HPV11 E6 was performed by polymerase chain reaction (PCR), while the viral DNA loads were measured by quantitative real-time PCR. Cytokine levels were determined by enzyme-linked immunosorbent assays. The CMV UL75 was detected more frequently in the blood of patients with AAA than in the blood of healthy volunteers (32.7% vs. 6.3%, p < .0001). Neither EBV LMP-1 nor HPV6 E6 was found in blood and aortic wall biopsies, while the HPV11 E6 was detected in 36.4% of AAA walls. The CMV infection in patients with AAA was associated with an increased risk of hypertension and coronary artery disease (OR, 9.057; 95% CI, 1.141-71.862; p = .037; and OR, 2.575; 95% CI, 1.002-6.615; p = .049, respectively). Additionally, CMV-infected patients with AAA had higher tumor necrosis factor-α levels compared with noninfected subjects (p = .017). Our findings suggest that CMV infection can stimulate local inflammation in the aorta but is not a direct cause of most abdominal aortic aneurysms.
腹主动脉瘤（AAA）是一种具有多种遗传和环境危险因素的多因素疾病，但AAA形成和进展的确切机制仍未完全清楚。本研究调查了巨细胞病毒（CMV）、爱泼斯坦-巴尔病毒（EBV）以及6型和11型乳头瘤病毒（HPV6和HPV11）的感染频率、它们对心血管疾病临床表现的影响，以及它们与AAA患者和健康志愿者炎症的可能关联。通过聚合酶链反应（PCR）对CMV UL75、EBV LMP-1、HPV6和HPV11 E6进行基因分型，同时通过定量实时PCR测量病毒DNA载量。通过酶联免疫吸附测定法测定细胞因子水平。与健康志愿者血液相比，AAA患者血液中CMV UL75的检测频率更高（32.7%对6.3%，p<0.0001）。在血液和主动脉壁活检中均未发现EBV LMP-1和HPV6 E6，而在36.4%的AAA壁中检测到HPV11 E6。AAA患者的CMV感染与高血压和冠状动脉疾病风险增加相关（OR分别为9.057；95%CI为1.141 - 71.862；p = 0.037；以及OR为2.575；95%CI为1.002 - 6.615；p = 0.049）。此外，与未感染的受试者相比，CMV感染的AAA患者肿瘤坏死因子-α水平更高（p = 0.017）。我们的研究结果表明，CMV感染可刺激主动脉局部炎症，但不是大多数腹主动脉瘤的直接病因。
