Ninnemann J L, Good R A
Transplantation. 1975 Jan;19(1):42-7. doi: 10.1097/00007890-197501000-00007.
In confirmation of prior studies, fragments of testicular interstitial (Leydig) cell tumors from BALB/c mice were found to be transplantable to H-2d (BALB/c and DBA/2) or allogeneic H-2q (DBA/1 and BUB/BnJ) recipients after organ culture, whereas noncultured fragments were accepted only in H-2d recipients. Lymphocyte cytotoxicity assays showed that while transplantability was altered by the organ culture process, tissue immunogenicity in vitro was unchanged. Cell suspensions from the same tumor were also found to be transplantable to both H-2d and H-2q recipients and occasionally even to H-2a and H-2k recipients, irrespective of whether the cells were obtained from fragments previously organ-cultured or from dispersed fresh tissue. We suggest, therefore, that rather than modifying cell surface antigens, it is possible that organ culture explanation serves to (1) allow free dispersal of the tumor cells which leads to progressive tumor growth, and/or (2) raise the ratio of dead to living cells in the transplanted fragments, which has previously been shown to direct host immune responses toward enhancement.
与先前的研究结果一致，发现经器官培养后，来自BALB/c小鼠的睾丸间质（莱迪希）细胞瘤碎片可移植到H-2d（BALB/c和DBA/2）或同种异体H-2q（DBA/1和BUB/BnJ）受体，而未经培养的碎片仅能被H-2d受体接受。淋巴细胞细胞毒性试验表明，虽然移植能力因器官培养过程而改变，但体外组织免疫原性未变。还发现来自同一肿瘤的细胞悬液可移植到H-2d和H-2q受体，甚至偶尔可移植到H-2a和H-2k受体，无论这些细胞是从先前经器官培养的碎片中获得还是从分散的新鲜组织中获得。因此，我们认为，器官培养的作用可能不是修饰细胞表面抗原，而是（1）使肿瘤细胞能够自由分散，从而导致肿瘤逐渐生长，和/或（2）提高移植碎片中死细胞与活细胞的比例，先前的研究表明这会引导宿主免疫反应向增强方向发展。
