Ansari N H, Bhatnagar A, Fulep E, Khanna P, Srivastava S K
Department of Ophthalmology, University of Texas Medical Branch, Galveston 77555.
Res Commun Chem Pathol Pharmacol. 1994 Apr;84(1):93-104.
Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman-2-carboxylic acid (Trolox) provided good protection against 50 mM glucose-induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose+antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.
利用连接通用C型接口和电荷耦合器件（CCD）相机的倒置显微镜，对在50 mM葡萄糖中培养的大鼠晶状体进行了高血糖诱导白内障形成的研究。采集数字图像，并通过定量透射光来确定晶状体的不透明度。抗氧化剂，丁基羟基甲苯（BHT）和6-羟基-2,5,7,8-四甲基苯并二氢吡喃-2-羧酸（Trolox）在长达8天的时间里为大鼠晶状体提供了良好的保护，防止50 mM葡萄糖诱导的白内障形成。50 mM葡萄糖+抗氧化剂组的山梨醇水平比50 mM葡萄糖组高出约1.5 mM倍。这些结果除了进一步证明氧化损伤是糖诱导白内障形成的主要机制外，还表明Trolox或相关的两亲性化合物可用于预防糖尿病性白内障。
