Kodentsova V M, Vrzhesinskaia O A, Nagaĭtseva Iu A
Vopr Pitan. 1993 Mar-Apr(2):55-8.
The authors propose a rapid method permitting riboflavin quality control after its addition to dishes. The method involves acid hydrolysis of the product using riboflavin-binding apoprotein. The assay requires no enzymatic hydrolysis and takes 1 hour. It correlates with a routine dithionite technique (r = 0.992), which tends to overestimate the results by 24%. In view of the method efficacy it is recommended for riboflavin assay in vitamin-enriched foodstuffs.
作者们提出了一种快速方法,可在向菜肴中添加核黄素后对其进行质量控制。该方法涉及使用核黄素结合脱辅基蛋白对产品进行酸水解。该测定无需酶水解,耗时1小时。它与常规连二亚硫酸盐技术相关(r = 0.992),常规技术往往会使结果高估24%。鉴于该方法的有效性,推荐用于富含维生素食品中的核黄素测定。