Hunt T K, Linsey M, Grislis H, Sonne M, Jawetz E
Ann Surg. 1975 Jan;181(1):35-9. doi: 10.1097/00000658-197501000-00009.
Wound infections were studied in rabbits using two standard inocula (approximately equal to 10-4 and approximately equal to 10-6) of Pseudomonas aeruginosa injected into subcutaneous wound dead space made by implantation of standard wire mesh cylinders. The inoculation was done on the fourth day after implantation of the cylinders in animals kept from the day of implantation in atmospheres of 12%, 21%, or 45% oxygen content. Samples of wound fluid (0.2 ml) were removed for quantitative culture just before inoculation and 3, 7, 14, and 21 days later. No positive cultures resulted from samples taken before inoculation. One uninoculated wound served as a control in each animal. None of these control wounds became infected. Culture counts were significantly highest in the anoxic group and lowest in the hyperoxic group. Established infections were significantly lowest in the hyperoxics and highest in the hypoxics. The percent of wounds showing a significant culture count showed a similar trend. The mechanisms of this effect is not known, but a possible mechanism lies in the relative inability of leucocytes to kill this bacterium under hypoxic conditions.
在兔子身上研究伤口感染情况，使用两种标准接种物（约等于10⁻⁴和约等于10⁻⁶）的铜绿假单胞菌注入由植入标准金属丝网圆柱体形成的皮下伤口死腔。在植入圆柱体后的第四天进行接种，这些动物从植入当天起分别饲养在氧气含量为12%、21%或45%的环境中。在接种前以及接种后3天、7天、14天和21天，采集伤口液体样本（0.2毫升）进行定量培养。接种前采集的样本没有培养出阳性结果。每只动物有一个未接种的伤口作为对照。这些对照伤口均未感染。培养计数在缺氧组显著最高，在高氧组最低。已形成的感染在高氧组显著最低，在低氧组最高。显示有显著培养计数的伤口百分比呈现类似趋势。这种效应的机制尚不清楚，但一种可能的机制在于白细胞在缺氧条件下相对无法杀死这种细菌。
