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负压伤口治疗可降低铜绿假单胞菌的活力，并在兔耳生物膜感染模型中促进伤口愈合。

Negative pressure wound therapy reduces the motility of Pseudomonas aeruginosa and enhances wound healing in a rabbit ear biofilm infection model.

作者信息

Guoqi Wang, Zhirui Li, Song Wang, Tongtong Li, Lihai Zhang, Licheng Zhang, Peifu Tang

机构信息

Department of Orthopedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, People's Republic of China.

Medical College, Nankai University, Tianjin, 300071, People's Republic of China.

出版信息

Antonie Van Leeuwenhoek. 2018 Sep;111(9):1557-1570. doi: 10.1007/s10482-018-1045-5. Epub 2018 Feb 21.

DOI:10.1007/s10482-018-1045-5
PMID:29468490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6097727/
Abstract

Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model. Swimming, swarming and twitching motility were significantly inhibited by NP (- 125 and - 200 mmHg) compared with atmospheric pressure (p = 0.05). Virulence factors and biofilm components were quantified in NPWT and gauze treated groups using a rabbit ear biofilm model. Biofilm structure was studied with fluorescence microscopy and scanning electron microscopy. Additionally, viable bacterial counts and histological wound healing parameters were measured. Compared with the control, NPWT treatment resulted in a significant reduction in expression of all virulence factors assayed including exotoxin A, rhamnolipid and elastase (p = 0.01). A significant reduction of biofilm components (eDNA) (p = 0.01) was also observed in the NPWT group. The reduction of biofilm matrix was verified by fluorescence- and scanning electron-microscopy. NPWT lead to better histologic parameters (p = 0.01) and decreased bacterial counts (p = 0.05) compared with the control. NPWT treatment was demonstrated to be an effective strategy to reduce virulence factors and biofilm components, which may explain the increased wound healing observed.

摘要

已知铜绿假单胞菌的运动性、毒力因子和生物膜对伤口愈合有害。负压伤口治疗（NPWT）对铜绿假单胞菌的疗效在体外或体内研究都很少。本研究评估了负压（NP）对体外铜绿假单胞菌运动性的影响，以及NPWT对体内毒力因子和生物膜的影响。使用体外模型在不同水平的NP（大气压、-75、-125、-200 mmHg）下对铜绿假单胞菌的运动性进行定量。与大气压相比，NP（-125和-200 mmHg）显著抑制了游动、群集和颤动运动（p = 0.05）。使用兔耳生物膜模型对NPWT组和纱布治疗组中的毒力因子和生物膜成分进行定量。用荧光显微镜和扫描电子显微镜研究生物膜结构。此外，还测量了活菌计数和组织学伤口愈合参数。与对照组相比，NPWT治疗导致所检测的所有毒力因子（包括外毒素A、鼠李糖脂和弹性蛋白酶）的表达显著降低（p = 0.01）。在NPWT组中也观察到生物膜成分（胞外DNA）显著减少（p = 0.01）。通过荧光显微镜和扫描电子显微镜证实了生物膜基质的减少。与对照组相比，NPWT导致更好的组织学参数（p = 0.01）和细菌计数减少（p = 0.05）。NPWT治疗被证明是一种减少毒力因子和生物膜成分的有效策略，这可能解释了观察到的伤口愈合增加的现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/52e757ace9c2/10482_2018_1045_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/bd9e0dfe862c/10482_2018_1045_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/cdbdf493d9cd/10482_2018_1045_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/52e757ace9c2/10482_2018_1045_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/bd9e0dfe862c/10482_2018_1045_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/f30846450632/10482_2018_1045_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/60a2364b2598/10482_2018_1045_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/0d199e77ec5a/10482_2018_1045_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/6c35fefd964b/10482_2018_1045_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/6b37d1cdd6ee/10482_2018_1045_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/e8acfb767b47/10482_2018_1045_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/08d041f411ff/10482_2018_1045_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/cdbdf493d9cd/10482_2018_1045_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/331c/6097727/52e757ace9c2/10482_2018_1045_Fig10_HTML.jpg

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