Kitada M, Horie T, Awazu S
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
Biochem Pharmacol. 1994 Jul 5;48(1):93-9.
Chemiluminescence was observed in a rat heart mitochondrial suspension containing NADH, FeC1(3) and doxorubicin (Adriamycin) (DXR). There was good correlation between the total intensity of chemiluminescence and the total amount of thiobarbituric acid reactive substances (TBARS) produced during DXR redox cycling. Thus, the chemiluminescence was shown to be associated with lipid peroxidation. The chemiluminescence was quenched by superoxide dismutase (SOD), suggesting that superoxide anion radicals contributed to its production. Upon addition of 1,4-diazabicyclo[2,2,2]-octane (DABCO), a singlet oxygen emission enhancer, to the mitochondrial suspension emitting the chemiluminescence, the chemiluminescence intensity increased transiently, indicating the involvement of singlet oxygen. Furthermore, spectral analysis of the chemiluminescence showed it to be due to singlet oxygen and excited carbonyls.
在含有烟酰胺腺嘌呤二核苷酸（NADH）、氯化铁（FeC1₃）和阿霉素（多柔比星）（DXR）的大鼠心脏线粒体悬浮液中观察到了化学发光现象。化学发光的总强度与DXR氧化还原循环过程中产生的硫代巴比妥酸反应性物质（TBARS）的总量之间存在良好的相关性。因此，化学发光被证明与脂质过氧化有关。超氧化物歧化酶（SOD）可淬灭化学发光，这表明超氧阴离子自由基参与了其产生过程。向发出化学发光的线粒体悬浮液中加入单线态氧发射增强剂1,4 - 二氮杂双环[2,2,2]辛烷（DABCO）后，化学发光强度会短暂增加，这表明单线态氧参与其中。此外，化学发光的光谱分析表明其源于单线态氧和激发态羰基。
