Fukuda F, Kitada M, Horie T, Awazu S
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
J Pharm Pharmacol. 1995 Mar;47(3):246-9. doi: 10.1111/j.2042-7158.1995.tb05788.x.
A rat heart mitochondrial suspension was incubated with doxorubicin, FeCl3 and NADH. Fluorescent substances and high molecular weight protein aggregates were observed in the mitochondrial membranes upon the formation of thiobarbituric acid-reactive substances. Since both fluorescent substances and high molecular weight protein aggregates are retained in mitochondrial membranes, they can be of use in the clarification of the site of doxorubicin-induced lipid peroxidation.
