从大鼠肝脏分离的粗糙型和光滑型微粒体中凝血酶原的生物学和免疫学活性

Biological and immunological activity of prothrombin in rough and smooth microsomes isolated from rat liver.

Janson T L, Helgeland L

Biochim Biophys Acta. 1975 Feb 27;379(2):598-605. doi: 10.1016/0005-2795(75)90166-x.

Abstract

The presence of biological and immunological activity of prothrombin in sonicates from rough and smooth microsomes has been investigated. 2. Biological activity of prothrombin was detected in both the rough and smooth microsomal fraction. The specific and the total activity of prothrombin in the sonicates from smooth microsomes were 3-4-fold higher than in the corresponding fraction from rough microsomes. 3. Prothrombin could be identified in both microsomal fractions by double immunodiffusion. 4. The presence of a macromolecular inhibitor of blood coagulation in the microsomes is reported.

摘要

已经研究了来自糙面微粒体和滑面微粒体的超声提取物中凝血酶原的生物学和免疫学活性。2. 在糙面微粒体和滑面微粒体部分均检测到凝血酶原的生物学活性。滑面微粒体超声提取物中凝血酶原的比活性和总活性比糙面微粒体相应部分高3至4倍。3. 通过双向免疫扩散可在两个微粒体部分中鉴定出凝血酶原。4. 报道了微粒体中存在一种血液凝固的大分子抑制剂。