High-performance liquid chromatographic assay for ibuprofen in whole blood using solid-phase extraction.

作者信息

Sochor J, Klimes J, Zahradnícek M, Sedlácek J

机构信息

Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.

出版信息

J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):282-6. doi: 10.1016/0378-4347(94)00018-2.

A precise, accurate, reproducible one-step method for the high-performance chromatographic determination of ibuprofen in whole blood is described. Samples were, after haemolysis, prepared by solid-phase extraction. Analyses were performed using reversed-phase chromatography on a Separon SGX C18 column with a mobile phase of methanol-water (pH 3) and ultraviolet detection at 222 nm. The method was used for pharmacokinetic studies in rabbits.