高效液相色谱法测定红细胞和血浆中的布洛芬。
Determination of ibuprofen in erythrocytes and plasma by high performance liquid chromatography.
作者信息
Sochor J, Klimes J, Sedlácek J, Zahradnícek M
机构信息
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Králové, Czech Republic.
出版信息
J Pharm Biomed Anal. 1995 Jun;13(7):899-903. doi: 10.1016/0731-7085(95)01306-6.
A high-performance liquid chromatographic (HPLC) method is described for the determination of ibuprofen in isolated erythrocytes and plasma. Before HPLC analysis ibuprofen was isolated by liquid-liquid extraction from these biological matrices; methylene chloride proved to be the best of the organic solvents tested. For the sample of erythrocytes it was necessary to carry out haemolysis prior to their extraction. HPLC was performed on a C-18 column with a mobile phase of methanol-water (220:100, v/v) acidified with perchloric acid to pH 3. Ultraviolet detection was at 222 nm. This method has been applied to the quantification of ibuprofen in rabbit erythrocytes and plasma for a pharmacokinetics study.
描述了一种用于测定分离的红细胞和血浆中布洛芬的高效液相色谱（HPLC）方法。在进行HPLC分析之前，通过液-液萃取从这些生物基质中分离出布洛芬；二氯甲烷被证明是所测试的有机溶剂中效果最佳的。对于红细胞样品，在萃取之前必须进行溶血处理。HPLC在C-18柱上进行，流动相为用高氯酸酸化至pH 3的甲醇-水（220:100，v/v）。紫外检测波长为222 nm。该方法已应用于兔红细胞和血浆中布洛芬的定量分析，用于一项药代动力学研究。
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