Reynolds B L, Rother U A, Rother K O
Infect Immun. 1975 May;11(5):944-8. doi: 10.1128/iai.11.5.944-948.1975.
Salmonella typhimurium C5 is under normal conditions (physiological saline containing 0.002 M Mg2+) resistant to the action of antibody and complement (C). It becomes sensitive, however, when suspended in tris(hydroxymethyl)-aminomethane buffer (Reynolds and Pruul, 1971; Reynolds and Rowley, 1969). The interaction of complement components with this strain sensitized with specific antibody has been studied to identify the intermediate step at which inhibition occurs. The components C1 yields C2 react normally, as has been shown by lysis of complement-treated cells incubated with complement in ethylenediaminetetraacetic acid. Also, the reaction of C3 can be demonstrated by positive immune adherence and agglutination with anti-C3. The complement-treated cells do not, however, react with rabbit C6 to 9 or rabbit serum lacking C6 in tris(hydroxymethyl)aminomethan buffer. We conclude from these date that C5 can not react effectively under normal conditions. In contrast, if bacteria-antibody complexes are pretreated with rabbit serum lacking C6 in tris(hydroxymethyl)aminomethane buffer, they are readily lysed by incubation with C6 to 9. Thus, C5 can react with the bacterial surface in tris(hydroxymethyl)-aminomethane buffer.
鼠伤寒沙门氏菌C5在正常条件下（含0.002 M Mg2+的生理盐水）对抗体和补体（C）的作用具有抗性。然而，当悬浮于三（羟甲基）氨基甲烷缓冲液中时（雷诺兹和普鲁尔，1971；雷诺兹和罗利，1969），它会变得敏感。已经研究了补体成分与用特异性抗体致敏的该菌株之间的相互作用，以确定抑制发生的中间步骤。如在乙二胺四乙酸中与补体一起孵育的经补体处理的细胞的裂解所显示的，成分C1产生C2的反应正常。此外，C3的反应可以通过阳性免疫黏附和抗C3凝集来证明。然而，在三（羟甲基）氨基甲烷缓冲液中，经补体处理的细胞不与兔C6至9或缺乏C6的兔血清反应。从这些数据我们得出结论，C5在正常条件下不能有效反应。相反，如果在三（羟甲基）氨基甲烷缓冲液中用缺乏C6的兔血清预处理细菌-抗体复合物，它们在与C6至9一起孵育时很容易被裂解。因此，C5可以在三（羟甲基）氨基甲烷缓冲液中与细菌表面反应。
