Kuwabara S
J Biol Chem. 1975 Apr 10;250(7):2563-8.
The complete amino acid sequence of tuberculin-active protein from tubercle bacilli was determined. The sequences of residues of tryptic, chymotryptic, and peptic peptides were established by Edman degradation and dansylation (5-dimethylaminonaphthalene-1-sulfonyl) and by digestion with carboxypeptidases A and B. Certain peptides were further degraded using partial acid hydrolysis. The aspartyl and glutamyl residues at positions 70 and 73 are present as amides. There is one intrachain disulfide bridge linking residues 27 and 59; there are no free sulfhydryls.
已确定结核杆菌中结核菌素活性蛋白的完整氨基酸序列。通过埃德曼降解法、丹磺酰化法（5-二甲基氨基萘-1-磺酰化）以及用羧肽酶A和B消化，确定了胰蛋白酶、胰凝乳蛋白酶和胃蛋白酶肽段的残基序列。某些肽段通过部分酸水解进一步降解。第70位和第73位的天冬氨酰和谷氨酰残基以酰胺形式存在。有一个链内二硫键连接第27位和第59位残基；不存在游离巯基。
