Pérez-Bendito D, Gómez-Hens A, Gaikwad A
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Clin Chem. 1994 Aug;40(8):1489-93.
Kinetic methodology was applied to the direct determination of abused drugs (amphetamines, cocaine, and cannabinoids) in urine by stopped-flow fluorescence polarization immunoassay (SF-FPIA). This technique provides analytical data within a few seconds by measuring the variation of polarized fluorescence with time during development of immunochemical reactions. Methods based on this principle are particularly suitable for routine screening of these drugs in urine, being more expeditious than conventional FPIA methods. The dynamic ranges of the calibration curves were 20-300 micrograms/L for d,l-amphetamine, 15-300 micrograms/L for benzoylecgonine (a cocaine metabolite), and 10-400 micrograms/L for 11-nor-delta 8-tetrahydrocannabinol-9-carboxylic acid (a cannabinoid metabolite). The detection limits and within- and between-assay precision were better than those provided by conventional FPIA. Analytical recoveries ranged between 97.5% for d,l-amphetamine and 102.4% for the cannabinoid metabolite. The results for the three analytes were consistent with those obtained by conventional FPIA.
采用动力学方法，通过停流荧光偏振免疫分析法（SF-FPIA）直接测定尿液中的滥用药物（苯丙胺类、可卡因和大麻素）。该技术通过在免疫化学反应过程中测量偏振荧光随时间的变化，在几秒钟内提供分析数据。基于这一原理的方法特别适用于尿液中这些药物的常规筛查，比传统的FPIA方法更迅速。校准曲线的动态范围为：d，l-苯丙胺为20 - 300微克/升，苯甲酰芽子碱（一种可卡因代谢物）为15 - 300微克/升，11-去甲-δ8-四氢大麻酚-9-羧酸（一种大麻素代谢物）为10 - 400微克/升。检测限以及批内和批间精密度均优于传统FPIA方法。分析回收率在d，l-苯丙胺的97.5%至大麻素代谢物的102.4%之间。三种分析物的结果与传统FPIA法获得的结果一致。
