Schwarzhoff R, Cody J T
Drug Testing Division, Armstrong Laboratory, Brooks Air Force Base, Texas 78235.
J Anal Toxicol. 1993 Jan-Feb;17(1):14-7. doi: 10.1093/jat/17.1.14.
A variety of chemical agents were evaluated to determine their effects on fluorescence polarization immunoassays for drugs of abuse. Sixteen different agents, at concentrations up to 10%, were tested against urine assays for cannabinoids, cocaine (metabolite), amphetamines, opiates, phencyclidine (PCP), and barbiturates. The potential to cause both false positive and false negative results was evaluated, and assays were performed one and seven days after sample adulteration to simulate different collection/testing formats. All six drug assays were susceptible to one or more adulterating agents, but the degree varied considerably between assays. The cannabinoid assay was most susceptible to adulterant-induced false negative results, and the barbiturate assay was most susceptible to false positive results. The remaining assays demonstrated relatively few, but characteristic effects, some of which were attributable to drug degradation and others to assay interference. Although the results of pH measurement on adulterated samples verified its utility in identifying some samples adulterated with interfering agents, other adulterants that cause substantial effects would not be identified by pH measurements alone.
评估了多种化学试剂，以确定它们对滥用药物荧光偏振免疫分析的影响。针对尿液中大麻素、可卡因（代谢物）、苯丙胺、阿片类药物、苯环己哌啶（PCP）和巴比妥类药物的检测，测试了16种不同的试剂，浓度最高达10%。评估了导致假阳性和假阴性结果的可能性，并在样本掺假后1天和7天进行检测，以模拟不同的采集/检测形式。所有六种药物检测都易受一种或多种掺假试剂的影响，但不同检测之间的程度差异很大。大麻素检测最易受掺假剂导致的假阴性结果影响，巴比妥类药物检测最易受假阳性结果影响。其余检测显示出相对较少但具有特征性的影响，其中一些归因于药物降解，另一些归因于检测干扰。尽管对掺假样本进行pH测量的结果证实了其在识别一些被干扰剂掺假的样本中的作用，但仅通过pH测量无法识别其他会产生重大影响的掺假剂。
