Frank M M, Gaither T
Immunology. 1970 Dec;19(6):975-81.
Sheep erythrocytes sensitized with rabbit γG anti-Forssman haemolysin may be haemolysed more efficiently and to a greater extent by limited amounts of guinea-pig complement components than are cells sensitized with γM anti-Forssman haemolysin. This apparent difference, noted in all components titrations, was two-fold or greater in titrations of C2 and components following C2 in the haemolytic sequence. Neither site to site transfer of C2 nor AC142 could account for the 100 per cent greater haemolytic efficiency of C2 in haemolytic assays. These results indicate that immunoglobulin class plays a more complex role in immune haemolysis than simple initiation of complement fixation and suggest that γG complement fixing sites may be more efficient than γM sites in producing biological damage.
用兔γG抗福斯曼溶血素致敏的绵羊红细胞，与用γM抗福斯曼溶血素致敏的细胞相比，在有限量豚鼠补体成分作用下，可能会更高效、更大量地发生溶血。在所有补体成分滴定实验中都观察到了这种明显差异，在溶血序列中C2及C2之后的成分滴定中，这种差异达两倍或更大。C2的位点间转移和AC142都无法解释溶血试验中C2溶血效率高出100%的现象。这些结果表明，免疫球蛋白类别在免疫溶血中所起的作用比单纯启动补体固定更为复杂，提示γG补体固定位点在产生生物损伤方面可能比γM位点更高效。
