Volodin A A, Smirnova H A, Bocharova T N
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
FEBS Lett. 1994 Jul 25;349(1):65-8. doi: 10.1016/0014-5793(94)00640-7.
Some fluorescein derivatives attached to the 5'-end of oligonucleotides stimulate recA protein-oligonucleotide binding. The complex formation at near stoichiometric DNA/protein ratios is demonstrated for 18-bases-long oligonucleotides. The complexes with dye-labeled oligonucleotides are shown to be active in the reaction of homologous strand exchange. The strand exchange reaction in the presence of adenosine-5'-O-(3-thiotriphosphate) proceeds with the formation of a stable complex of recA protein with the double stranded oligonucleotide, which is a product of the strand exchange. The displaced single-stranded oligonucleotide is shown to be bound weakly.
一些连接在寡核苷酸5'端的荧光素衍生物可刺激RecA蛋白与寡核苷酸的结合。对于18个碱基长的寡核苷酸，在接近化学计量的DNA/蛋白质比例下证明了复合物的形成。带有染料标记寡核苷酸的复合物在同源链交换反应中显示出活性。在腺苷-5'-O-(3-硫代三磷酸)存在下的链交换反应会形成RecA蛋白与双链寡核苷酸的稳定复合物，该双链寡核苷酸是链交换的产物。被置换的单链寡核苷酸显示出弱结合。
