大肠杆菌RecA蛋白与单链寡脱氧核糖核苷酸的相互作用。
Interaction of the RecA protein of Escherichia coli with single-stranded oligodeoxyribonucleotides.
作者信息
Bianco P R, Weinstock G M
机构信息
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225, USA.
出版信息
Nucleic Acids Res. 1996 Dec 15;24(24):4933-9. doi: 10.1093/nar/24.24.4933.
Abstract
The RecA protein of Escherichia coli performs a number of ATP-dependent, in vitro reactions and is a DNA-dependent ATPase. Small oligodeoxyribonucleotides were used as DNA cofactors in a kinetic analysis of the ATPase reaction. Polymers of deoxythymidilic acid as well as oligonucleotides of mixed base composition stimulated the RecA ATPase activity in a length-dependent fashion. Both the initial rate and the extent of the reaction were affected by chain length. Full activity was seen with chain lengths > or = 30 nt. Partial activity was seen with chain lengths of 15-30 nt. The lower activity of shorter oligonucleotides was not simply due to a reduced affinity for DNA, since effects of chain length on KmATP and the Hill coefficient for ATP hydrolysis were also observed. The results also suggested that single-stranded DNA secondary structure frequently affects the ATPase activity of RecA protein with oligodeoxyribonucleotides.
摘要
大肠杆菌的RecA蛋白可进行多种依赖ATP的体外反应，是一种依赖DNA的ATP酶。在ATP酶反应的动力学分析中，小的寡脱氧核糖核苷酸被用作DNA辅因子。脱氧胸苷酸聚合物以及混合碱基组成的寡核苷酸以长度依赖的方式刺激RecA ATP酶活性。反应的初始速率和程度均受链长影响。链长≥30 nt时可见完全活性。链长为15 - 30 nt时可见部分活性。较短寡核苷酸活性较低并非仅仅是由于对DNA的亲和力降低，因为还观察到链长对KmATP和ATP水解的希尔系数有影响。结果还表明，单链DNA二级结构常影响RecA蛋白与寡脱氧核糖核苷酸的ATP酶活性。
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本文引用的文献
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Homologous recognition and triplex formation promoted by RecA protein between duplex oligonucleotides and single-stranded DNA.RecA蛋白促进双链寡核苷酸与单链DNA之间的同源识别和三链体形成。
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Homologous recognition promoted by RecA protein via non-Watson-Crick bonds between identical DNA strands.RecA蛋白通过相同DNA链之间的非沃森-克里克键促进同源识别。
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Interactions of three strands in joints made by RecA protein.RecA 蛋白形成的接头中三条链的相互作用。
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Why does RecA protein hydrolyse ATP?RecA蛋白为何会水解ATP？
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Efficient interaction of recA protein with fluorescent dye-labeled oligonucleotides.RecA蛋白与荧光染料标记的寡核苷酸的有效相互作用。
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Biochemistry of homologous recombination in Escherichia coli.大肠杆菌中同源重组的生物化学
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tif-1 mutation alters polynucleotide recognition by the recA protein of Escherichia coli.tif-1突变改变了大肠杆菌recA蛋白对多核苷酸的识别。
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recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.大肠杆菌的RecA蛋白促进分支迁移，这是DNA链交换中一个动力学上不同的阶段。
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Hydrolysis of nucleoside triphosphates catalyzed by the recA protein of Escherichia coli. Hydrolysis of UTP.大肠杆菌recA蛋白催化的核苷三磷酸水解。UTP的水解。
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