Quigley H, Pease M E, Thibault D
Glaucoma Service, Wilmer Institute, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
Graefes Arch Clin Exp Ophthalmol. 1994 May;232(5):257-61. doi: 10.1007/BF00194473.
Elastin fibers in the lamina cribrosa of glaucoma eyes have a curled appearance when the tissue is fixed at atmospheric pressure. To evaluate the effect of preparation conditions on elastin appearance, we set the eye pressure at one of four levels during tissue fixation in 18 glaucoma and 16 normal eyes. Glaucomatous damage was associated with an abnormal appearance of elastin at each pressure level. The majority of glaucoma eyes (11/18) had curled elastin profiles. In glaucoma eyes with definite nerve damage, elastin was graded moderately or severely abnormal in 47% (7/15), while in normal controls the rate of similar curling was 6% (1/16). The severity of abnormal elastin appearance was lower in glaucoma eyes that were fixed and frozen at higher intraocular pressures, but the difference was not statistically significant.
当在大气压下固定组织时，青光眼患者筛板中的弹性纤维呈现卷曲外观。为了评估制备条件对弹性纤维外观的影响，我们在18只青光眼眼和16只正常眼中的组织固定过程中将眼压设定为四个水平之一。在每个压力水平下，青光眼性损伤都与弹性纤维的异常外观有关。大多数青光眼眼（11/18）有卷曲的弹性纤维轮廓。在有明确神经损伤的青光眼眼中，47%（7/15）的弹性纤维被评为中度或重度异常，而在正常对照眼中，类似卷曲的发生率为6%（1/16）。在较高眼压下固定和冷冻的青光眼眼中，弹性纤维外观异常的严重程度较低，但差异无统计学意义。
