对映体亮氨酸脑啡肽的保守和新颖结构特征。亮氨酸脑啡肽对映体L-酪氨酸-甘氨酸-甘氨酸-L-苯丙氨酸-L-亮氨酸和D-酪氨酸-甘氨酸-甘氨酸-D-苯丙氨酸-D-亮氨酸的X射线晶体分析。

Conserved and novel structural characteristics of enantiomorphic Leu-enkephalin. X-ray crystal analysis of Leu-enkephalin enantiomer, L-Tyr-Gly-Gly-L-Phe-L-Leu and D-Tyr-Gly-Gly-D-Phe-D-Leu.

作者信息

Doi M, Ishibe A, Shinozaki H, Urata H, Inoue M, Ishida T

机构信息

Osaka University of Pharmaceutical Sciences, Japan.

出版信息

Int J Pept Protein Res. 1994 Apr;43(4):325-31. doi: 10.1111/j.1399-3011.1994.tb00526.x.

DOI:10.1111/j.1399-3011.1994.tb00526.x

PMID:8045677

Abstract

The crystal of the Leu-enkephalin racemate (L-Tyr-Gly-Gly-L-Phe-L-Leu and D-Tyr-Gly-Gly-D-Phe-D-Leu) was obtained as a centrosymmetric space group. Crystal data: C28H37N5O7 x 1.5H2O, Mw = 582.6, triclinic, space group P1, a = 11.176(3), b = 16.115(3), c = 10.204(4) A, alpha = 92.41(3), beta = 104.86(2), gamma = 85.35(2)degrees, V = 1770(1)A3, Z = 2; F(000) = 640, mu(CuK alpha) = 6.50 cm-1, D chi = 1.081 g cm-3. The structure was determined by X-ray diffraction. The conformation of the Leu-enkephalin racemate was classified into the extended form which has been often observed in natural enkephalin. The symmetry-related molecules were connected by hydrogen bonds and arranged in an antiparallel fashion. The molecular packing showed a sheet structure similar to that of natural enkephalin.

摘要

亮氨酸脑啡肽外消旋体（L-酪氨酸-甘氨酸-甘氨酸-L-苯丙氨酸-L-亮氨酸和D-酪氨酸-甘氨酸-甘氨酸-D-苯丙氨酸-D-亮氨酸）的晶体以中心对称空间群形式获得。晶体数据：C28H37N5O7·1.5H2O，分子量 = 582.6，三斜晶系，空间群P1，a = 11.176(3)，b = 16.115(3)，c = 10.204(4) Å，α = 92.41(3)，β = 104.86(2)，γ = 85.35(2)°，V = 1770(1) Å3，Z = 2；F(000) = 640，μ(CuKα) = 6.50 cm-1，Dχ = 1.081 g cm-3。结构通过X射线衍射确定。亮氨酸脑啡肽外消旋体的构象被归类为在天然脑啡肽中经常观察到的伸展形式。对称相关分子通过氢键连接并以反平行方式排列。分子堆积呈现出与天然脑啡肽类似的片状结构。