Sanchez M S, Ford C W, Yancey R J
Upjohn Company, Kalamazoo, MI 49001.
J Dairy Sci. 1994 May;77(5):1251-8. doi: 10.3168/jds.S0022-0302(94)77064-8.
A bovine blood polymorphonuclear neutrophil system was used to determine the ability of cytokines, alone or in combination with various antibiotics, to enhance the intracellular killing of Staphylococcus aureus sequestered within these phagocytes. Pretreatment of blood polymorphonuclear neutrophils with human recombinant tumor necrosis factor-alpha, bovine macrophage supernatant tumor necrosis factor-alpha, or human recombinant interleukin-1 beta enhanced the killing of S. aureus by the phagocytes compared with that of untreated polymorphonuclear neutrophils. This enhancement was dose-dependent and required 30 min of contact between the cytokine and the blood polymorphonuclear neutrophils. However, mammary gland polymorphonuclear neutrophils, macrophages, and blood monocytes showed no enhanced killing activity following pretreatment with tumor necrosis factor. Pretreatment of polymorphonuclear neutrophils with tumor necrosis factor and subsequent exposure to various antibiotics after infection with S. aureus enhanced intracellular bacterial killing by certain antibiotics, including paldimycin, rifampin, and ciprofloxacin (antibiotics that normally kill intracellular S. aureus). However, no enhancement occurred with antibiotics that do not normally kill intracellular S. aureus. Cytokine therapy may be a useful adjunct to antibiotic therapy in the treatment of bovine staphylococcal infection and mastitis.
使用牛血多形核中性粒细胞系统来确定细胞因子单独或与各种抗生素联合使用时，增强这些吞噬细胞内对金黄色葡萄球菌的杀伤能力。与未处理的多形核中性粒细胞相比，用人重组肿瘤坏死因子-α、牛巨噬细胞上清液肿瘤坏死因子-α或人重组白细胞介素-1β预处理血多形核中性粒细胞可增强吞噬细胞对金黄色葡萄球菌的杀伤作用。这种增强呈剂量依赖性，且细胞因子与血多形核中性粒细胞之间需要接触30分钟。然而，乳腺多形核中性粒细胞、巨噬细胞和血单核细胞在用肿瘤坏死因子预处理后未显示出增强的杀伤活性。用肿瘤坏死因子预处理多形核中性粒细胞，随后在感染金黄色葡萄球菌后接触各种抗生素，可增强某些抗生素（包括帕地霉素、利福平和环丙沙星，这些抗生素通常可杀死细胞内的金黄色葡萄球菌）的细胞内细菌杀伤作用。然而，对于通常不能杀死细胞内金黄色葡萄球菌的抗生素则没有增强作用。细胞因子疗法可能是治疗牛葡萄球菌感染和乳腺炎时抗生素治疗的有用辅助手段。
