Sanchez M S, Ford C W, Yancey R J
Upjohn Company, Kalamazoo, MI 49001.
J Dairy Sci. 1994 May;77(5):1259-66. doi: 10.3168/jds.S0022-0302(94)77065-X.
The mouse model was used to determine the efficacy of the cytokine, tumor necrosis factor-alpha, and antibiotic in treatment of experimentally induced staphylococcal mastitis. Recombinant human tumor necrosis factor-alpha alone administered to the mammary glands of lactating mice recruited significantly more polymorphonuclear neutrophils into the gland by 4 h posttreatment than did the untreated control. One hundred times less recombinant mouse tumor necrosis factor-alpha than human tumor necrosis factor-alpha was required to enhance the killing of Staphylococcus aureus within the gland. Human tumor necrosis factor-alpha effectively enhanced the killing of the bacteria when it was administered 4 to 0 h prior to infection, but not 4 h after infection. When mice were first pretreated with tumor necrosis factor-alpha, infected, and then treated with antibiotics (ciprofloxacin and pirlimycin, but not cloxacillin), the combination of antibiotic and cytokine significantly reduced the number of bacteria within the gland compared with that for mice treated with antibiotic alone, cytokine alone, or placebo. Recombinant tumor necrosis factor-alpha may be an effective adjunct to antimicrobial therapy in treatment of staphylococcal mastitis in the bovine.
该小鼠模型用于确定细胞因子肿瘤坏死因子-α和抗生素在治疗实验性诱导的葡萄球菌性乳腺炎中的疗效。与未治疗的对照组相比，在治疗后4小时，单独向泌乳小鼠乳腺施用重组人肿瘤坏死因子-α可使更多的多形核中性粒细胞募集到乳腺中。增强乳腺内金黄色葡萄球菌的杀伤作用所需的重组小鼠肿瘤坏死因子-α的量比重组人肿瘤坏死因子-α少100倍。当在感染前4至0小时施用时，人肿瘤坏死因子-α可有效增强细菌的杀伤作用，但在感染后4小时施用则无效。当小鼠先用肿瘤坏死因子-α预处理、感染，然后用抗生素（环丙沙星和吡利霉素，但不包括氯唑西林）治疗时，与单独用抗生素、单独用细胞因子或安慰剂治疗的小鼠相比，抗生素和细胞因子的组合可显著减少乳腺内的细菌数量。重组肿瘤坏死因子-α可能是治疗牛葡萄球菌性乳腺炎的抗菌治疗的有效辅助药物。
