Brayman A A, Miller M W
Department of Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642.
J Acoust Soc Am. 1994 Jun;95(6):3666-8. doi: 10.1121/1.409937.
In polystyrene exposure vessels, a substantial fraction of suspended P388 cells was lysed by exposure to cw, 1-MHz ultrasound (5 W cm-2 spatial peak intensity, 5 min) in stationary vessels or those subjected to a small number of half rotations. Lysis was increased significantly by two half-rotations during exposure relative to stationary vessels. Lysis in stationary exposure vessels is generally considered to be minimal; it is shown that approximately 40% cell lysis occurs in 15%-20% of the stationary tubes. This result appears to relate to the distribution of random defects on the exposure vessel walls.
在聚苯乙烯暴露容器中，相当一部分悬浮的P388细胞在固定容器或进行少量半旋转的容器中，通过暴露于连续波1兆赫超声（空间峰值强度5瓦/平方厘米，5分钟）而被裂解。与固定容器相比，暴露期间进行两次半旋转可显著增加裂解率。固定暴露容器中的裂解通常被认为是最小的；结果表明，约40%的细胞裂解发生在15%-20%的固定管中。这一结果似乎与暴露容器壁上随机缺陷的分布有关。
