Church C C, Flynn H G, Miller M W, Sacks P G
Ultrasound Med Biol. 1982;8(3):299-309. doi: 10.1016/0301-5629(82)90036-9.
This paper is an attempt to explain the need to rotate a polystyrene tube containing a cell suspension in order to obtain cell lysis. Calculations, based on known physical laws, were performed in order to determine the important forces on cells and bubbles and the movements and interactions which these forces are likely to cause. These calculations support the following conclusions: (1) in the absence of rotation, cells and bubbles larger than resonance size are trapped at pressure minima while bubbles smaller than resonance size are trapped at pressure maxima, (2) at 1 W/cm2 with rotation, lysis is caused by cells sweeping through arrays of trapped small bubbles, (3) at higher intensities lysis is caused by both trapped and non-trapped small bubbles.
本文旨在解释旋转装有细胞悬液的聚苯乙烯管以实现细胞裂解的必要性。基于已知物理定律进行了计算，以确定作用于细胞和气泡的重要力以及这些力可能导致的运动和相互作用。这些计算支持以下结论：（1）在不旋转的情况下，大于共振尺寸的细胞和气泡被困在压力最小值处，而小于共振尺寸的气泡被困在压力最大值处；（2）在1 W/cm²旋转条件下，细胞扫过被困小气泡阵列会导致细胞裂解；（3）在更高强度下，被困和未被困的小气泡都会导致细胞裂解。
