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谷氨酸转运体GLT-1在大鼠和猕猴视网膜中的定位。

Localization of the glutamate transporter GLT-1 in rat and macaque monkey retinae.

作者信息

Rauen T, Kanner B I

机构信息

Max-Planck-Institut für Hirnforschung, Neuroanatomische Abteilung, Frankfurt am Main, FRG.

出版信息

Neurosci Lett. 1994 Mar 14;169(1-2):137-40. doi: 10.1016/0304-3940(94)90375-1.

DOI:10.1016/0304-3940(94)90375-1
PMID:8047270
Abstract

Antibodies directed against a glutamate transporter (GLT-1) purified from rat brain were applied to cryostat sections of rat and macaque monkey retinae. In the brain, GLT-1 expression is found mainly in astrocytes, and therefore it has been suggested that GLT-1 may be a glutamate transporter specific to glial cells. However, in the rat retina, cones and two distinct cone bipolar cell types were strongly immunoreactive. In the monkey retina, flat midget bipolars and one diffuse bipolar cell type (DB2)), were found to be labelled. Müller cells or astrocytes, the neuroglial cells of rat and monkey retinae, were not GLT-1-immunoreactive.

摘要

将针对从大鼠脑纯化的谷氨酸转运体(GLT-1)的抗体应用于大鼠和猕猴视网膜的低温切片。在脑中,GLT-1表达主要见于星形胶质细胞,因此有人提出GLT-1可能是一种胶质细胞特异性的谷氨酸转运体。然而,在大鼠视网膜中,视锥细胞和两种不同类型的视锥双极细胞呈强免疫反应性。在猕猴视网膜中,扁平侏儒双极细胞和一种弥散双极细胞类型(DB2)被发现有标记。大鼠和猕猴视网膜的神经胶质细胞,即米勒细胞或星形胶质细胞,无GLT-1免疫反应性。

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