Grünert U, Martin P R, Wässle H
Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
J Comp Neurol. 1994 Oct 22;348(4):607-27. doi: 10.1002/cne.903480410.
Transfer of visual information from photoreceptors to ganglion cells within the retina is mediated by specialized groups of bipolar cells. At least 10 different morphological types of bipolar cells have been distinguished in Golgi studies of primate retina. In the present study, bipolar cell populations in the macaque monkey retina were identified by their differential immunoreactivity to a spectrum of antibody markers. This enabled their spatial density and photoreceptor connections to be analysed. An antibody against the beta isozyme of protein kinase C (PKCA beta) labelled many cone bipolar cells. Invaginating (presumed ON) cone bipolar cells and rod bipolar cells were preferentially labelled with a monoclonal antibody raised against rabbit olfactory bulb. Flat (presumed OFF) bipolar cells were labelled with an antiserum against the glutamate transporter protein (GLT-1). Different populations of diffuse cone bipolar cells, which contact 5-10 cones, could be distinguished. The GLT-1 antiserum preferentially labelled the flat diffuse bipolar cell type DB2 (Boycott and Wässle, 1991, Eur. J. Neurosci. 3:1069-1088) as well as flat midget bipolar cells. Antibodies to calbindin (CaBP D-28K) labelled the flat diffuse bipolar cell type DB3 and (possibly) the invaginating diffuse bipolar cell type DB5. An antibody against the alpha isozyme of PKC labelled an invaginating diffuse bipolar cell type (DB4) as well as rod bipolar cells. Comparison of the spatial density of cone bipolar cell populations with that of photoreceptors suggests that each bipolar cell class provides a complete coverage of the cone array (each cone is contacted by at least one member of every bipolar cell class). These results support the classification scheme of Boycott and Wässle (1991) by showing that different diffuse bipolar cell classes express different patterns of immunoreactivity, and they reinforce the view that different spatial and temporal components of the signal from the photoreceptor array are processed in parallel within the primate retina.
视觉信息在视网膜内从光感受器向神经节细胞的传递是由特定的双极细胞群介导的。在对灵长类视网膜的高尔基研究中，已区分出至少10种不同形态类型的双极细胞。在本研究中，通过猕猴视网膜双极细胞群体对一系列抗体标记物的不同免疫反应性来识别它们。这使得能够分析它们的空间密度和与光感受器的连接。一种针对蛋白激酶C（PKC）β同工酶的抗体标记了许多视锥双极细胞。内陷型（推测为ON型）视锥双极细胞和视杆双极细胞被一种针对兔嗅球产生的单克隆抗体优先标记。扁平型（推测为OFF型）双极细胞被一种针对谷氨酸转运蛋白（GLT - 1）的抗血清标记。可以区分接触5 - 10个视锥的不同群体的弥散型视锥双极细胞。GLT - 1抗血清优先标记扁平弥散双极细胞类型DB2（Boycott和Wässle，1991年，《欧洲神经科学杂志》3:1069 - 1088）以及扁平侏儒双极细胞。抗钙结合蛋白（CaBP D - 28K）抗体标记了扁平弥散双极细胞类型DB3以及（可能）内陷弥散双极细胞类型DB5。一种针对PKCα同工酶的抗体标记了一种内陷弥散双极细胞类型（DB4）以及视杆双极细胞。视锥双极细胞群体的空间密度与光感受器的空间密度比较表明，每个双极细胞类别对视锥阵列提供了完整的覆盖（每个视锥至少与每个双极细胞类别的一个成员接触）。这些结果通过表明不同的弥散双极细胞类别表达不同的免疫反应性模式，支持了Boycott和Wässle（1991年）的分类方案，并且它们强化了这样一种观点，即来自光感受器阵列的信号的不同空间和时间成分在灵长类视网膜内并行处理。
