Moleón I, González T, Machín R, Molina J R, García C A
Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba.
Rev Latinoam Microbiol. 1993 Jul-Sep;35(3):309-14.
Toxoplasmosis is a widely distributed disease with prevalence rates ranging between 40 and 80% in different parts of the world. The diagnosis of congenital toxoplasmosis constitutes a health problem since intra-uterine infection can lead to undesirable effects on the fetus. Immunoenzymatic methods are the techniques of choice for the serologic diagnosis of toxoplasmosis. The present paper describes an indirect sandwich ELISA with the murine anti-P30, the main surface antigen of Toxoplasma gondii, monoclonal antibody as the first antibody fixed to the polystyrene 96 wells plate for the capture of P30 molecule from T. gondii extracts and to detect the presence of human anti-P30 IgG. Serum samples from 42 pregnant women were studied and compared to results obtained with a commercial kit (Plastelia Toxo IgG, Institute Pasteur, Lille, France) for the same purpose, in which 83.4% of the serum samples were positives. Our results demonstrated a significant correlation (p < 0.001; r = 0.84) with those obtained with the commercial kit. We conclude that the IgG anti-P30 method should be very useful for screening of seroconversion in pregnant women not sensitized by T. gondii and to evaluate epidemiologically the prevalence rate infection by this parasite which can cause and transmit this disease subclinically.
弓形虫病是一种广泛分布的疾病，在世界不同地区的患病率在40%至80%之间。先天性弓形虫病的诊断构成一个健康问题，因为宫内感染会对胎儿产生不良影响。免疫酶法是弓形虫病血清学诊断的首选技术。本文描述了一种间接夹心酶联免疫吸附测定法（ELISA），该方法以小鼠抗P30（刚地弓形虫主要表面抗原）单克隆抗体作为第一抗体固定在聚苯乙烯96孔板上，用于从刚地弓形虫提取物中捕获P30分子并检测人抗P30 IgG的存在。研究了42名孕妇的血清样本，并将结果与使用商业试剂盒（法国里尔巴斯德研究所的Plastelia Toxo IgG）获得的结果进行比较，该商业试剂盒检测的血清样本阳性率为83.4%。我们的结果表明与使用商业试剂盒获得的结果具有显著相关性（p < 0.001；r = 0.84）。我们得出结论，IgG抗P30方法对于筛查未被刚地弓形虫致敏的孕妇的血清转化以及从流行病学角度评估这种可导致并亚临床传播该疾病的寄生虫的感染患病率应该非常有用。
