Golkar Majid, Azadmanesh Kayhan, Khalili Ghader, Khoshkholgh-Sima Baharak, Babaie Jalal, Mercier Corinne, Brenier-Pinchart Marie-Pierre, Fricker-Hidalgo Hélène, Pelloux Hervé, Cesbron-Delauw Marie-France
Molecular Parasitology Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran.
Diagn Microbiol Infect Dis. 2008 May;61(1):31-9. doi: 10.1016/j.diagmicrobio.2007.09.003. Epub 2008 Jan 31.
Indirect immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays (ELISAs) with a recombinant GRA6 protein of Toxoplasma gondii were developed and evaluated for accurate diagnosis of recently acquired infection in pregnant women. According to the results from Toxoplasma serodiagnostic tests, women were classified into 3 groups representing acute (group I), chronic (group II), or no Toxoplasma infection (group III). To discriminate group I from group II sera, the GRA6-IgG-ELISA reached sensitivity and specificity of 87.5% and 94.1%, respectively. Although 22 (91.7%) of 24 group I sera were positive by the GRA6-IgM-ELISA, only 1 (2.9%) of 34 group II sera scored positive. The GRA6-IgM-ELISA displayed a meaningful correlation with Vidas Toxo IgM and exhibited higher specificity (97.1%) than Euroimmun IgM ELISA (88.2%) (Euroimmun, Lübeck, Germany) for detection of recent infection. These results demonstrate that IgG and IgM ELISA with rGRA6 are useful to identify and discriminate recent from past Toxoplasma infection in pregnant women.
开发了针对刚地弓形虫重组GRA6蛋白的间接免疫球蛋白G（IgG）和IgM酶联免疫吸附测定（ELISA），并对其进行评估，以准确诊断孕妇近期获得的感染。根据弓形虫血清学诊断测试的结果，将女性分为3组，分别代表急性感染（I组）、慢性感染（II组）或无弓形虫感染（III组）。为了区分I组和II组血清，GRA6-IgG-ELISA的敏感性和特异性分别达到87.5%和94.1%。虽然24份I组血清中有22份（91.7%）通过GRA6-IgM-ELISA呈阳性，但34份II组血清中只有1份（2.9%）呈阳性。GRA6-IgM-ELISA与Vidas Toxo IgM显示出有意义的相关性，并且在检测近期感染方面比Euroimmun IgM ELISA（88.2%，Euroimmun，德国吕贝克）具有更高的特异性（97.1%）。这些结果表明，rGRA6的IgG和IgM ELISA有助于识别和区分孕妇近期与既往的弓形虫感染。
