Subbotina Iu L, Levenson V I
Zh Mikrobiol Epidemiol Immunobiol. 1980 Jun(6):76-82.
Stable erythrocytic diagnostic reagents have been prepared on the basis of formalinized erythrocytes coated with purified IgG, IgM, IgA ans sIgA with the aim of the quantitative determination of immunoglobulins in the hemaglutination inhibition (HAI) test. The sensitivity of the HAI test in the quantitative determination of immunoglobulins is 50--100 times higher, but its accuracy is lower than those of radial immunodiffusion (RID). This is the cause of a high variability in indices of serum immunoglobulin levels in healthy persons. The parallel study of sera with altered immunoglobulin levels by means of the HAI test and RID revealed, as a rule, a good correlation of their results. The proteolytic breakdown and aggregation of immunoglobulins produced similar changes in the results of the HAI test and RID. The possibility of using the HAI test for the study of local immune response in infections diseases has been shown by study of coprofiltrates obtained from dysentery patients (an increase in the level of sIgA has been detected).
以包被有纯化IgG、IgM、IgA和sIgA的甲醛化红细胞为基础，制备了稳定的红细胞诊断试剂，用于在血凝抑制（HAI）试验中定量测定免疫球蛋白。HAI试验在免疫球蛋白定量测定中的灵敏度高50 - 100倍，但其准确性低于放射免疫扩散（RID）。这就是健康人血清免疫球蛋白水平指标变异性高的原因。通过HAI试验和RID对免疫球蛋白水平改变的血清进行平行研究，结果通常显示两者具有良好的相关性。免疫球蛋白的蛋白水解降解和聚集在HAI试验和RID结果中产生了类似的变化。通过对痢疾患者粪便滤液的研究（检测到sIgA水平升高）表明，HAI试验可用于研究感染性疾病中的局部免疫反应。
