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碳酸氢盐对淋病奈瑟菌生长的影响:气态二氧化碳环境的替代

Effects of bicarbonate on growth of Neisseria gonorrhoeae: replacement of gaseous CO2 atmosphere.

作者信息

Talley R S, Baugh C L

出版信息

Appl Microbiol. 1975 Apr;29(4):469-71. doi: 10.1128/am.29.4.469-471.1975.

Abstract

The effect of NaHCO2 on the growth of Neisseria gonorrhoeae cultures was studied in a liquid and a semisolid growth medium. With a broth culture, NaHCO3 (0.009 M) greatly reduced the lag phase and also increased the total growth. The same concentration of bicarbonate supported rapid growth when added to the semisolid medium if the plates were individually incubated in sealed plastic bags. In a container with a large air space, a higher concentration of NaHCO3 was necessary to support growth. The assimilation of 14C-labeled NaHO3 by growing cultures was also investigated.

摘要

在液体和半固体生长培养基中研究了碳酸氢钠(NaHCO₂)对淋病奈瑟菌培养物生长的影响。在肉汤培养中,碳酸氢钠(0.009M)大大缩短了延迟期,并且还增加了总生长量。如果将平板分别放在密封的塑料袋中培养,当向半固体培养基中添加相同浓度的碳酸氢盐时,可支持快速生长。在具有大空气空间的容器中,需要更高浓度的碳酸氢钠来支持生长。还研究了生长中的培养物对¹⁴C标记的碳酸氢钠(NaHO₃)的同化作用。

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本文引用的文献

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Comparison of atmospheric conditions for culture of clinical specimens of Neisseria gonorrhoeae.
Appl Microbiol. 1973 Oct;26(4):466-9. doi: 10.1128/am.26.4.466-469.1973.

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